1、下載安裝MrBayes

http://nbisweden.github.io/MrBayes/

git clone --depth=1 https://github.com/NBISweden/MrBayes.git

cd?MrBayes

./configure --prefix=/work/workspace/username/program/MrBayes

ln -s? /work/workspace/username/program/MrBayes/bin/mb?/work/workspace/username/bin

2、fasta 轉(zhuǎn)?nexus

https://www.ebi.ac.uk/Tools/msa/clustalo/

將sequence.nexus上傳到文件夾Dir4MrBayes

3、設(shè)置突變模型invgamma，1M次Bayes建樹

mb

MrBayes > Execute? sequence.nexus;

MrBayes > prset statefreqpr=fixed(empirical); lset rates=invgamma;

MrBayes > mcmcp ngen=1000000 nchains=4 printfreq=100 samplefreq=100 burnin=200;

MrBayes >?mcmc;

MrBayes >?sump;

MrBayes >?sumt;

MrBayes >?quit

4、展示最后結(jié)果FigTree

下載FigTree?https://github.com/rambaut/figtree/releases

運(yùn)行FigTree v1.4.4

載入sequence.nexus.con.tre

Appearance -> 設(shè)置分支格式, Line Weight: 2

Tip Labels -> 設(shè)置字體格式，F(xiàn)ont: Arial, size: 14

Branch Labels -> 設(shè)置分支標(biāo)簽格式，Display: prob(percent)? Font: Arial, size: 14,?

Scale Axis -> 設(shè)置圖例標(biāo)尺格式，F(xiàn)ont size: 14,?Line Weight: 2

File -> Export PDF

Tips: 修改環(huán)形展示方式

Layout: Polar tree layout