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植物器官生長由基因遺傳決定的其大小和形狀。在植物研究中，如何精細(xì)調(diào)控器官生長以實現(xiàn)明確的模式是一個引人入勝但在很大程度上尚未解決的問題。我們利用擬南芥花瓣來研究植物器官生長的遺傳控制，通過篩選花瓣特異的促生長轉(zhuǎn)錄因子兔耳(RBE)的靶標(biāo)，確定了兩個密切相關(guān)的U-box E3連接酶PUB25和PUB26是重要的生長調(diào)節(jié)因子。
圖 PUB25/26突變體和超表達(dá)植物的表型鑒定
我們發(fā)現(xiàn)PUB25在花瓣發(fā)育中受RBE以時空特異性的方式直接控制，并且是介導(dǎo)RBE在花瓣生長中作用的主要靶點(diǎn)。我們還發(fā)現(xiàn)PUB25和PUB26通過限制細(xì)胞增殖期來抑制花瓣生長，并且它們的調(diào)節(jié)似乎獨(dú)立于其他與生長控制有關(guān)的植物E3連接酶基因。PUB25和PUB26是第一批在植物生長控制中發(fā)揮作用的U-box E3連接酶。
此外，由于PUB25和PUB26還被發(fā)現(xiàn)在植物逆境反應(yīng)中起著至關(guān)重要的作用，因此它們可能是整合發(fā)育和環(huán)境信號以平衡植物生長和防御的關(guān)鍵樞紐。
Plant organs often grow into a genetically determined size and shape. How organ growth is finely regulated to achieve a well defined pattern is a fascinating, but largely unresolved, question in plant research.
We utilised the Arabidopsis petal to study the genetic control of plant organ growth, and identify two closely related U‐box E3 ligases?PUB25?and?PUB26?as important growth regulators by screening the targets of the petal‐specific growth‐promoting transcription factor RABBIT EARS (RBE).
We showed that?PUB25?is directly controlled by RBE in petal development in a spatial‐ and temporal‐specific manner and acts as a major target to mediate RBE’s function in petal growth. We also showed that?PUB25?and?PUB26?repress petal growth by restricting the period of cell proliferation, and their regulation appears to be independent of other plant E3 ligase genes implicated in growth control.
PUB25 and PUB26 are among the first U‐box E3 ligases shown to function in plant growth control. Furthermore, as they were also found to play a vital role in plant stress responses, PUB25 and PUB26 may act as a key hub to integrate developmental and environmental signals for balancing growth and defence in plants.
來源：
https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.16885?af=R
通訊作者：Tengbo Huang，深圳大學(xué)生命科學(xué)與海洋學(xué)院廣東省植物表觀遺傳學(xué)重點(diǎn)實驗室，深圳
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