今天看到一個(gè)3分+非OA期刊出版了一篇相對比較容易操作的純生信數(shù)據(jù)挖掘的文章（一般科研小白都會(huì)操作），這篇文章下載了相關(guān)TCGA數(shù)據(jù)庫的miRNA和mRNA~差異分析～功能分析（GO、KEGG富集分析、PPI分析）～篩選關(guān)鍵的miRNA和mRNA~生存分析～GEO數(shù)據(jù)進(jìn)行驗(yàn)證。這個(gè)非OA期刊就是：Expert Review of Anticancer Therapy，非OA不需要版面費(fèi)，影響因子：3.573，中科院最新分區(qū)：3區(qū)，不在中科院預(yù)警名單內(nèi)，審稿周期：2到3個(gè)月。
這篇文章于2021年1月28日在Expert Review of Anticancer Therapy出版，文章題目如下：
miRNA and mRNA expression profiling reveals potential biomarkers for metastatic cutaneous melanoma
文章摘要：
Purpose
This study aims to uncover potential biomarkers associated with cutaneous melanoma (CM) metastasis.
Methods
The mRNA and microRNA (miRNA) expression data from the metastatic CM and non-metastatic CM population were obtained from The Cancer Genome Atlas database. Functional analysis, protein-protein interaction (PPI), and survival analysis were performed for differentially expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs). The interaction between DEmRNAs and DEmiRNAs was analysed. The expression of several key DEmRNAs and DEmiRNAs was validated by Gene Expression Omnibus datasets.
Results
Overall, 1172 DEmRNAs and 26 DEmiRNAs were identified from metastatic and non-metastatic CM. Cytokine-cytokine receptor interaction and chemokine signalling pathway were key pathways. CXCR1, CXCR2, CXCR4, CCR1, CCR2, and CCR5 were hub genes in the PPI network. Among these, miR-29c-3p, miR-100-5p, miR-150-5p, and miR-150-3p were not only diagnostic biomarkers, but also related to survival time. miR-203a-3p interacted with CCR5 and LIFR, while miR-224-5p was strongly associated with CXCR4. LIFR, CXCR1, CXCR2, CXCR4, CCR1, CCR2, and CCR5 were enriched in the cytokine-cytokine receptor interaction pathway. The levels of seven DEmRNAs (CXCR1, CXCR2, CXCR4, CCR1, CCR2, CCR5, and LIFR) and two DEmiRNAs (miR-203a-3p and miR-224-5p) were validated using the GSE65568 and GSE109244 datasets, respectively.
Conclusion
Our findings may provide novel biomarkers for CM metastasis.
總結(jié)：
相比于那些結(jié)合研究熱點(diǎn)構(gòu)建模型的文章，miRNA和mRNA~差異分析～功能分析（GO、KEGG富集分析、PPI分析）～篩選關(guān)鍵的miRNA和mRNA~生存分析～GEO數(shù)據(jù)進(jìn)行驗(yàn)證這種分析思路真的是太簡單了。簡單歸簡單，關(guān)鍵還要看選擇了對的期刊，遇見了對的審稿人。
