我最近在整理Bioconductor一系列包，但是每次都會遇到一些bug，這次是在安裝R包時遇到問題，卡了5天，一直以為是自己設(shè)置的問題，今天終于解決了。
Bioconductor安裝的麻煩就在于，每個包依賴的包太多了，而且這次主要是很多包都依賴IRanges，這是基礎(chǔ)，不安裝很多包都用不了。 這個系列如果不設(shè)置鏡像（下載慢），不經(jīng)常更新（每次都提醒你更新），不嘗試多方檢索解決問題（有前人的經(jīng)驗(yàn)），可能要郁悶死了。
報錯的幾個包
TCGAbiolinks包
Iranges包
Biostrings包
這個報錯檢索了很久，各種嘗試設(shè)置也不行，我又要崩潰了。
不知是不是巧合，也是我自己能get到，每次我從檢索生信技能樹的教程得到啟發(fā)，崩潰幾天，然后解決，這次是這兩個推送：
【緊急通知】下載R包卻聯(lián)網(wǎng)失?。砍鯇W(xué)者的痛
BiocManager無法安裝R包
解決辦法
我采取的辦法就是卸載重裝，先在Rstudio中右下角工作環(huán)境中package處移除這個包，然后關(guān)閉Rstudio，再把下載目錄(就是你的包下載后存放的地方)下有關(guān)包的文件全部刪掉。
然后重啟Rstudio，輸入安裝代碼。如下：
options()$repos
options()$BioC_mirror
options(BioC_mirror="https://mirrors.ustc.edu.cn/bioc/")
options("repos" = c(CRAN="https://mirrors.tuna.tsinghua.edu.cn/CRAN/"))
options()$repos
options()$BioC_mirror
if (!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("IRanges")
library(IRanges)
BiocManager::install("Biostrings")
library(Biostrings)
然后包就下載成功，再次加載就沒有問題了。
附：報錯的一長串，一開始看到真的還挺慌的…………
反思：不知道是哪兒設(shè)置出了問題，或者就是下載目錄被動過。總之，R真的是應(yīng)了那句，遇事不決，重啟解決。
雞湯一句： 遇到問題不要沮喪，萬物皆有裂痕，那是光照進(jìn)來的地方。
Error: package or namespace load failed for ‘IRanges’ in namespaceExport(ns, exports):
出口不存在：diff.AtomicList, window<-.Vector, window<-.vector, window<-.factor, splitAsList, multisplit, solveUserSEW0, IRanges, solveUserSEW, successiveIRanges, slidingIRanges, breakInChunks, whichAsIRanges, asNormalIRanges, rangeComparisonCodeToLetter, IPos, NCList, NCLists, H2LGrouping, Dups, PartitioningByEnd, PartitioningByWidth, PartitioningMap, RangedData, RangedSelection, RangesList, IRangesList, RleViewsList, %over%, %within%, %outside%, %pover%, %pwithin%, %poutside%, mergeByOverlaps, findOverlapPairs, MaskCollection.show_frame, Mask, read.gapMask, read.agpMask, read.liftMask, read.rmMask, read.trfMask, successiveViews, slidingViews, LogicalList, IntegerList, NumericList, ComplexList, CharacterList, RawList, RleList, FactorList, DataFrameList, SplitDataFrameList, ManyToOneGrouping, ManyToManyGrouping, regroup, heads, tails, selectNearest, INCOMPATIBLE_ARANGES_MSG, extractListFragments, equisplit, ranges, rglist, mstack, mid, isNormal, whichFirstNotNormal, subject, ranges<-, Views, trim, subviews, viewApply, viewMins, viewMaxs, viewSums, viewMeans, viewWhichMins, viewWhichMaxs, viewRangeMins, viewRangeMaxs, nobj, grouplengths, members, vmembers, togroup, togrouplength, high2low, low2high, grouprank, togrouprank, mapOrder, findRange, splitRanges, extractList, columnMetadata, columnMetadata<-, universe, universe<-, nir_list, desc, desc<-, maskedwidth, maskedratio, findOverlaps, countOverlaps, overlapsAny, subsetByOverlaps, overlapsRanges, poverlaps, narrow, update_ranges, shift, resize, flank, reflect, promoters, restrict, threebands, reduce, gaps, disjoin, isDisjoint, disjointBins, reverse, coverage, cvg, slice, punion, pintersect, psetdiff, pgap, precede, follow, nearest, distance, distanceToNearest, tile, slidingWindows, ifelse2
Failed with error: ‘無法載入程輯包‘IRanges’’
