不一定,不同開發(fā)者的定義不同。
早上爬樓看到這個問題:
各位有個基礎(chǔ)問題我不是很明白,SAM文件中的MAPQ map質(zhì)量等于255究竟是什么意思,網(wǎng)上查了好多,包括官網(wǎng)的解釋都是not available,但是像STAR這種軟件給出的卻是unique map,那么not available和unique map之間有什么關(guān)系嗎?還是每個軟件對MAPQ數(shù)值的定義本身就不同?
我們知道,在cellranger中:
MAPQ adjustment
For reads that align to a single exonic locus but also align to 1 or more non-exonic loci, the exonic locus is prioritized and the read is considered to be confidently mapped to the exonic locus with MAPQ 255.
于是,一頓搜索操作,整理成ppt:

https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/algorithms/overview
http://www.acgt.me/blog/2014/12/16/understanding-mapq-scores-in-sam-files-does-37-42
https://www.biostars.org/p/178387/
https://www.biostars.org/p/372666/