先放上今天的筆記

Day 03.png

minicinda的下載

wget [https://mirrors.tuna.tsinghua.edu.cn/anaconda/miniconda/Miniconda3-latest-Linux-x86_64.sh](https://mirrors.tuna.tsinghua.edu.cn/anaconda/miniconda/Miniconda3-latest-Linux-x86_64.sh)

安裝

bash Mini----
source ~/.bashrc

使用minicoanda

conda list  #列表
conda search fastqc
conda install fastqc -y
conda remove fastqc -y

conda環(huán)境

conda info --envs
conda creat  -n rna-seq python=3 fastqc trimmomatic -y
conda activate rna-seq
conda deactivate

關于conda環(huán)境的構建，等實際操作項目時再進行學習

再搭建案例環(huán)境后輸入fastqc，以下顯示不知對不對

[wang@VM_16_12_centos biosoft]$ conda activate rna-seq
(rna-seq) [wang@VM_16_12_centos biosoft]$ fastqc
Exception in thread "main" java.awt.HeadlessException: 
No X11 DISPLAY variable was set, but this program performed an operation which requires it.
    at java.desktop/java.awt.GraphicsEnvironment.checkHeadless(GraphicsEnvironment.java:208)
    at java.desktop/java.awt.Window.<init>(Window.java:548)
    at java.desktop/java.awt.Frame.<init>(Frame.java:423)
    at java.desktop/java.awt.Frame.<init>(Frame.java:388)
    at java.desktop/javax.swing.JFrame.<init>(JFrame.java:180)
    at uk.ac.babraham.FastQC.FastQCApplication.<init>(FastQCApplication.java:63)
    at uk.ac.babraham.FastQC.FastQCApplication.main(FastQCApplication.java:338)