1. Cellranger的下載
此處以10X官網(wǎng)上最新的Cellranger版本為例下載，網(wǎng)址如下：
wget -O cellranger-6.1.2.tar.gz "https://cf.10xgenomics.com/releases/cell-exp/cellranger-6.1.2.tar.gz?Expires=1635685923&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cHM6Ly9jZi4xMHhnZW5vbWljcy5jb20vcmVsZWFzZXMvY2VsbC1leHAvY2VsbHJhbmdlci02LjEuMi50YXIuZ3oiLCJDb25kaXRpb24iOnsiRGF0ZUxlc3NUaGFuIjp7IkFXUzpFcG9jaFRpbWUiOjE2MzU2ODU5MjN9fX1dfQ__&Signature=IdVssu4uhoH2HQcJ6Fc66z~Wcf8x09lfKU0g2ARgKBjB11bc3IroxYh3ZeExzOJzZkTNjuIo94pRmjMZ9GjjIzOwteLBmKU83nA2JUbUPW28CAfvWFiuTDme94Ty68XUuYEXNPYJA88YP3OyMGgh2T4KKAdYRen2GDjmu~MvUZdNtOniTsWNbT0IQBeoGI8DE1Rcl1QLiK90uiCcw-aXOsTc~w4OQZiEUj0vd5N~KgpUWHdd4Nzy9Y0~SP~DBFkSYYcKEZ6qOUnq~BUDOq5QMcsPfz244WNaFfnUkLnF2tnzXQsjYvDm7obs2iWUGRXgnODcJ7B9UXRrLG5wDfdI7A__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"
下載至某一文件夾，本例中以下載至software文件夾為例。
cd ~/software
之后，進行解包、解壓縮。
tar -zxvf cellranger-6.1.2.tar.gz
2. 添加Cell Ranger到$PATH
export PATH=~/download/cellranger-6.1.2:$PATH
檢驗是否添加到$PATH:
which cellranger
輸出如下：
/home/user_name/software/cellranger-6.1.2/cellranger # user_name是所使用服務器用戶的名字
也可以添加到.bashrc，這樣下次登陸就不用再次export了，切換到家目錄下,
vi .bashrc
將下方這句添加到.bashrc文件中，
export PATH=~/download/cellranger-6.1.2:$PATH
更新一下.bashrc文件。
source .bashrc
3. 執(zhí)行 sitecheck
cellranger sitecheck > sitecheck.txt
發(fā)送sitecheck.txt到10X的支持團隊
cellranger upload your_email@email.com sitecheck.txt
輸出如下，
Getting upload information...
Uploading file...
Size: 17,676 bytes
100% [=================================>] 17,676 0.28Mb/s eta 0m 0s
Upload complete!
4. Cellranger試運行
cellranger testrun --id=tiny
試運行結果輸出如下，
Pipestance completed successfully!
