1. 首先下載IMvigor210CoreBiologies包得壓縮包文件，然后本地安裝
   這一步參考
   IMvigor210CoreBiologies包安裝指北 - 騰訊云開發(fā)者社區(qū)-騰訊云 (tencent.com)
   【R>>IMvigor210CoreBiologies】免疫反應 - 簡書 (jianshu.com)
   IMvigor210CoreBiologies的安裝以及數(shù)據(jù)獲取 - 簡書 (jianshu.com)

可以看到上述教程中的安裝難點均在于 DESeq這個包

我們從以下鏈接中下載對應壓縮包文件
Bioconductor - DESeq (tu-dortmund.de)

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最核心的一步來了?。。?！
使用devtools包中的 install_local函數(shù)強制安裝這個包

devtools::install_local('C:/Users/shihaosun/Desktop/DESeq_1.34.1.tar.gz',force = T)

── R CMD build ───────────────────────────────────────────────────────────
?  checking for file 'C:\AppData\Local\Temp\RtmpkBBtqZ\remotes110818515094\DESeq/DESCRIPTION' ... 
─  preparing 'DESeq':
?  checking DESCRIPTION meta-information ... 
─  cleaning src
?  checking vignette meta-information ...
─  checking for LF line-endings in source and make files and shell scripts
─  checking for empty or unneeded directories
─  building 'DESeq_1.34.1.tar.gz'
   
將程序包安裝入‘C:/Users/shihaosun/AppData/Local/R/win-library/4.2’
(因為‘lib’沒有被指定)
* installing *source* package 'DESeq' ...
** using staged installation
** libs
gcc  -I"C:/PROGRA~1/R/R-42~1.1/include" -DNDEBUG     -I"c:/rtools42/x86_64-w64-mingw32.static.posix/include"     -O2 -Wall  -std=gnu99 -mfpmath=sse -msse2 -mstackrealign  -c pval.c -o pval.o
pval.c: In function 'add_from_both_sides':
pval.c:26:11: warning: unused variable 'esttotalperlength' [-Wunused-variable]
   26 |    double esttotalperlength = total/2;
      |           ^~~~~~~~~~~~~~~~~
gcc -shared -s -static-libgcc -o DESeq.dll tmp.def pval.o -Lc:/rtools42/x86_64-w64-mingw32.static.posix/lib/x64 -Lc:/rtools42/x86_64-w64-mingw32.static.posix/lib -LC:/PROGRA~1/R/R-42~1.1/bin/x64 -lR
installing to C:/Users/shihaosun/AppData/Local/R/win-library/4.2/00LOCK-DESeq/00new/DESeq/libs/x64
** R
** inst
** byte-compile and prepare package for lazy loading
Warning message:
package 'locfit' was built under R version 4.2.2 
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
Warning: package 'locfit' was built under R version 4.2.2
** testing if installed package can be loaded from final location
Warning: package 'locfit' was built under R version 4.2.2
** testing if installed package keeps a record of temporary installation path
* DONE (DESeq)

可以看到全程無報錯 直接安裝。

包得加載也順利完成。

image.png

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小小記錄一下！