文獻(xiàn)DOI：10.1016/j.neuropharm.2016.12.022

文獻(xiàn)PMID：28025096

文獻(xiàn)原文鏈接：http://doi.org.sci-hub.tw/10.1016/j.neuropharm.2016.12.022

Early vs. late intervention of high fat/low dose streptozotocin treated C57Bl/6J mice with enalapril, α-lipoic acid, menhaden oil or their combination: Effect on diabetic neuropathy related endpoints

應(yīng)用依那普利、α-硫辛酸，鯡魚油或其組合早期和晚期干預(yù)高脂肪/低劑量鏈脲佐菌素處理的C57Bl / 6J小鼠：對糖尿病神經(jīng)病變相關(guān)終點(diǎn)的影響

【Abstract】

We have previously demonstrated that enalapril, α-lipoic acid and menhaden (fish) oil has potential as a treatment for diabetic peripheral neuropathy. In this study we sought to determine the efficacy of these treatments individually or in combination on multiple neuropathic endpoints in a high fat fed low dose streptozotocin treated mouse, a model of type 2 diabetes, following early or late intervention. Four or twelve weeks after the onset of hyperglycemia, diabetic mice were treated with enalapril, α-lipoic acid, menhaden oil or their combination for 12 weeks. Afterwards, endpoints including glucose tolerance, motor and sensory nerve conduction velocity, thermal nociception, and intraepidermal and cornea nerve fiber density was determined. Glucose clearance was impaired in diabetic mice and significantly improved only with combination treatment and early intervention. Diabetes caused steatosis, slowing of motor and sensory nerve conduction velocity, thermal hypoalgesia and reduction in intraepidermal and cornea nerve fiber density. Treating diabetic mice with enalapril, α-lipoic acid or menhaden oil partially protected diabetic mice from these deficits, whereas the combination of these three treatments was more efficacious following early or late intervention. These studies suggest that a combination therapy may be more effective for treating neural complications of type 2 diabetes.

摘要翻譯：

我們之前已經(jīng)證明，依那普利、α-硫辛酸和鯡魚（魚）油具有治療糖尿病周圍神經(jīng)病變的潛力。在本研究中，我們試圖在早期或晚期干預(yù)后，在高脂肪喂養(yǎng)的低劑量鏈脲佐菌素治療的小鼠（2型糖尿病模型）中單獨(dú)或組合地確定這些治療對多個神經(jīng)病學(xué)終點(diǎn)的功效。高血糖發(fā)作后4或12周，糖尿病小鼠用依那普利、α-硫辛酸、鯡魚油或它們的組合治療12周。然后，確定包括葡萄糖耐量、運(yùn)動和感覺神經(jīng)傳導(dǎo)速度、熱傷害感受以及表皮內(nèi)和角膜神經(jīng)纖維密度的終點(diǎn)。糖尿病小鼠的葡萄糖清除率受損，僅在聯(lián)合治療和早期干預(yù)時顯著改善。糖尿病引起脂肪變性、運(yùn)動和感覺神經(jīng)傳導(dǎo)速度減慢、熱痛覺減退和表皮內(nèi)和角膜神經(jīng)纖維密度減少。用依那普利、α-硫辛酸或鯡魚油治療糖尿病小鼠可部分保護(hù)糖尿病小鼠免于這些缺陷，而這三種治療的組合在早期或晚期干預(yù)后均更有效。這些研究表明聯(lián)合治療可能更有效地治療2型糖尿病的神經(jīng)并發(fā)癥。