（1）ensemble_vep 下載和安裝
http://asia.ensembl.org/info/docs/tools/vep/script/vep_download.html
git clone https://github.com/Ensembl/ensembl-vep
cd ensembl-vep
（2）perl 包安裝方式
http://www.cpan.org/modules/INSTALL.html
cpan App::cpanminus #基本用法
cpan DBI #安裝DBI，安裝過程中容易出現(xiàn)依賴庫不存在的問題，ln -s .so /.a

（3）安裝htslib 過程中出現(xiàn)問題
/usr/bin/ld： cant find -lcurl
解決方法：
/usr/lib64/curl 或者/usr/lib/curl

(base) [dujl@localhost lib64]$ ll *curl*
lrwxrwxrwx. 1 root root     16 Aug  1 23:31 libcurl.so.4 -> libcurl.so.4.3.0
-rwxr-xr-x. 1 root root 435192 Nov  5  2018 libcurl.so.4.3.0
(base) [dujl@localhost lib64]$  ln -s /usr/lib64/libcurl.so.4.3.0 ./libcurl.so
##創(chuàng)建 libcurl.so 連接
(base) [dujl@localhost lib64]$ ll *curl*
lrwxrwxrwx. 1 root root     27 Oct 19 10:07 libcurl.so -> /usr/lib64/libcurl.so.4.3.0
lrwxrwxrwx. 1 root root     16 Aug  1 23:31 libcurl.so.4 -> libcurl.so.4.3.0
-rwxr-xr-x. 1 root root 435192 Nov  5  2018 libcurl.so.4.3.0
(base) [dujl@localhost htslib]$ make
##不會報錯
(base) [dujl@localhost htslib]$ make test
.......
Number of tests:
    total   .. 154
    passed  .. 154
    failed  .. 0
(base) [dujl@localhost htslib] pwd 
~/Biosoftware/VEP/ensembl-vep/src

安裝以上內容后，perl INSTALL.pl 仍然會報錯，報錯內容如下：

(base) [dujl@localhost ensembl-vep]$ perl INSTALL.pl 
curl failed (000), trying to fetch using LWP::Simple
LWP::Simple failed (501), trying to fetch using HTTP::Tiny
ERROR: Failed last resort of using HTTP::Tiny to download https://raw.githubusercontent.com/Ensembl/ensembl-vep/release/101/modules/Bio/EnsEMBL/VEP/Constants.pm

查了別人的文檔，表示“ 安裝過程中經常會報錯，所以最好找個網(wǎng)絡好的時間安裝，比方說早上八九點，網(wǎng)絡不好報的錯誤大概如下，報這個錯誤之后，就重新開個中斷，重新安裝，多試幾次，最后應該可以成功”
https://www.cnblogs.com/afeiyuanda/p/13168166.html