在分子生物學(xué)中 我們會(huì)有將GB格式序列文件 轉(zhuǎn)換成 Fasta格式文件的需求，這里我們利用python腳本來解決這個(gè)問題。
GB格式文件和FASTA文件介紹
gb格式文件是GenBank的文件，用來保存序列的詳細(xì)信息。包含一個(gè)gene的名稱，編號(hào)，發(fā)現(xiàn)者，參考文獻(xiàn)，外顯子位置，編碼區(qū)序列，蛋白序列等等信息。
例如：
LOCUS NM_213806 849 bp mRNA linear MAM 24-SEP-2019
DEFINITION Sus scrofa Fas ligand (TNF superfamily, member 6) (FASLG), mRNA.
ACCESSION NM_213806
VERSION NM_213806.1
KEYWORDS RefSeq.
SOURCE Sus scrofa (pig)
ORGANISM Sus scrofa
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
Sus.
REFERENCE 1 (bases 1 to 849)
AUTHORS Lin F, Fu YH, Han J, Shen M, Du CW, Li R, Ma XS and Liu HL.
TITLE Changes in the expression of Fox O1 and death ligand genes during
follicular atresia in porcine ovary
JOURNAL Genet. Mol. Res. 13 (3), 6638-6645 (2014)
PUBMED 25177944
REMARK GeneRIF: Data suggest forkhead box protein O1 (FoxO1) involvement
in the regulation of TNF-related apoptosis-inducing ligand TRAIL
and Fas ligand FasL expression during follicular atresia.
Publication Status: Online-Only
REFERENCE 2 (bases 1 to 849)
AUTHORS Xie GH, Wang SJ, Wang Y, Zhang Y, Zhang HZ, Jin S, Wang QF, Liu ZC
and Ge HL.
TITLE Fas Ligand gene transfer enhances the survival of tissue-engineered
chondrocyte allografts in mini-pigs
JOURNAL Transpl. Immunol. 19 (2), 145-151 (2008)
PUBMED 18503890
REMARK GeneRIF: the result indicates that the expression of FasL by
chondrocytes is capable of inducing apoptosis of activated T cells
REFERENCE 3 (bases 1 to 849)
AUTHORS Chang HW, Jeng CR, Lin CM, Liu JJ, Chang CC, Tsai YC, Chia MY and
Pang VF.
TITLE The involvement of Fas/FasL interaction in porcine circovirus type
2 and porcine reproductive and respiratory syndrome virus
co-inoculation-associated lymphocyte apoptosis in vitro
JOURNAL Vet. Microbiol. 122 (1-2), 72-82 (2007)
PUBMED 17321702
REMARK GeneRIF: The expression of FAS and FAS ligand in splenic
macrophages co-infected with porcine circovirus 2 and porcine
reproductive and respiratory syndrome virus is reported
REFERENCE 4 (bases 1 to 849)
AUTHORS Tayade C, Black GP, Fang Y and Croy BA.
TITLE Differential gene expression in endometrium, endometrial
lymphocytes, and trophoblasts during successful and abortive embryo
implantation
JOURNAL J. Immunol. 176 (1), 148-156 (2006)
PUBMED 16365405
REFERENCE 5 (bases 1 to 849)
AUTHORS Bai L, Maedler K, Donath M and Tuch BE.
TITLE Expression of Fas but not Fas ligand on fetal pig beta cells
JOURNAL Xenotransplantation 11 (5), 426-435 (2004)
PUBMED 15303979
REMARK GeneRIF: FasL was not detected on fetal pig pancreatic cells but
could be induced on both beta and non-beta cells when the cells
were treated with IL1beta.
Erratum:[Xenotransplantation. 2016 Mar;23(2):171-2. PMID: 27106874]
REFERENCE 6 (bases 1 to 849)
AUTHORS Tsuyuki S, Kono M and Bloom ET.
TITLE Cloning and potential utility of porcine Fas ligand: overexpression
in porcine endothelial cells protects them from attack by human
cytolytic cells
JOURNAL Xenotransplantation 9 (6), 410-421 (2002)
PUBMED 12371937
REFERENCE 7 (bases 1 to 849)
AUTHORS Motegi-Ishiyama Y, Nakajima Y, Hoka S and Takagaki Y.
TITLE Porcine Fas-ligand gene: genomic sequence analysis and comparison
with human gene
JOURNAL Mol. Immunol. 38 (8), 581-586 (2002)
PUBMED 11792426
REFERENCE 8 (bases 1 to 849)
AUTHORS Muneta Y, Shimoji Y, Inumaru S and Mori Y.
TITLE Molecular cloning, characterization, and expression of porcine Fas
ligand (CD95 ligand)
JOURNAL J. Interferon Cytokine Res. 21 (5), 305-312 (2001)
PUBMED 11429161
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from AB027297.1.
##Evidence-Data-START##
Transcript exon combination :: AB027297.1, AF397407.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMN01893940, SAMN01915393
[ECO:0000348]
##Evidence-Data-END##
FEATURES Location/Qualifiers
source 1..849
/organism="Sus scrofa"
/mol_type="mRNA"
/db_xref="taxon:9823"
/chromosome="9"
/map="9"
gene 1..849
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/note="Fas ligand (TNF superfamily, member 6)"
/db_xref="GeneID:396726"
CDS 1..849
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/note="CD95 ligand; tumor necrosis factor (ligand)
superfamily, member 6; fas antigen ligand"
/codon_start=1
/product="tumor necrosis factor ligand superfamily member
6"
/protein_id="NP_998971.1"
/db_xref="GeneID:396726"
/translation="MQQPFNYPYPQIFWVDSSATSPWASPGSVFPCPASVPGRPGQRR
PPPPPPPPPPPPTLLPSRPLPPLPPPSLKKKRDHNAGLCLLVMFFMVLVALVGLGLGM
FQLFHLQKELTELRESASQRHTESSLEKQIGHPNLPSEKKELRKVAHLTGKPNSRSIP
LEWEDTYGIALVSGVKYMKGSLVINDTGLYFVYSKVYFRGQYCNNQPLSHKVYTRNSR
YPQDLVLMEGKMMNYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESKTF
FGLYKL"
mat_peptide 1..390
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/product="ADAM10-processed FasL form. {ECO:0000250}"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)"
mat_peptide 1..249
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/product="FasL intracellular domain. {ECO:0000250}"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)"
misc_feature 244..249
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/experiment="experimental evidence, no additional details
recorded"
/note="Cleavage, by SPPL2A. {ECO:0000250}; propagated from
UniProtKB/Swiss-Prot (Q9BEA8.1); cleavage site"
misc_feature 247..309
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1);
transmembrane region"
misc_feature 388..393
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/experiment="experimental evidence, no additional details
recorded"
/note="Cleavage, by ADAM10. {ECO:0000250}; propagated from
UniProtKB/Swiss-Prot (Q9BEA8.1); cleavage site"
mat_peptide 391..846
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/product="Tumor necrosis factor ligand superfamily member
6, soluble form. {ECO:0000250}"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)"
misc_feature 553..555
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/experiment="experimental evidence, no additional details
recorded"
/note="N-linked (GlcNAc...) asparagine. {ECO:0000255};
propagated from UniProtKB/Swiss-Prot (Q9BEA8.1);
glycosylation site"
misc_feature 751..753
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/experiment="experimental evidence, no additional details
recorded"
/note="N-linked (GlcNAc...) asparagine. {ECO:0000255};
propagated from UniProtKB/Swiss-Prot (Q9BEA8.1);
glycosylation site"
misc_feature 781..783
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/experiment="experimental evidence, no additional details
recorded"
/note="N-linked (GlcNAc...) asparagine. {ECO:0000255};
propagated from UniProtKB/Swiss-Prot (Q9BEA8.1);
glycosylation site"
exon 1..351
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/inference="alignment:Splign:2.1.0"
exon 352..397
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/inference="alignment:Splign:2.1.0"
exon 398..454
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/inference="alignment:Splign:2.1.0"
exon 455..849
/gene="FASLG"
/gene_synonym="CD95-L; FASL; TNFSF6"
/inference="alignment:Splign:2.1.0"
ORIGIN
1 atgcagcagc ccttcaatta cccatacccc caaatcttct gggtggacag cagtgctacc
61 tctccctggg cctccccagg ctcagtcttc ccctgtccag cttctgtgcc aggaaggcca
121 gggcaaagga ggccaccacc accaccgccg ccaccgccac caccaccaac actcctgcca
181 tcaagaccgc tgcctccact gccaccgcca tctctgaaga agaagaggga ccacaatgca
241 ggcctgtgtc tccttgtgat gttcttcatg gttctggtgg ccctggttgg attggggctg
301 gggatgtttc agctcttcca cctacagaag gagctgactg aactcagaga gtctgccagc
361 caaaggcata cagaatcatc tttggagaag caaataggtc accccaatct accctctgag
421 aaaaaggagc tgagaaaagt ggcccactta acaggcaagc ctaactcaag atccatccct
481 ctggaatggg aagacaccta tggaattgcc ttggtctctg gggtgaagta tatgaagggc
541 agccttgtga tcaatgacac tgggctgtat tttgtgtatt ccaaagtgta cttccggggt
601 cagtactgca acaaccagcc cctgagtcac aaggtataca caaggaactc taggtatccc
661 caggacctgg tgctgatgga gggaaagatg atgaactatt gcactactgg ccaaatgtgg
721 gcccgcagca gctacctggg ggctgtgttc aatctcacca gcgctgacca tttatatgtc
781 aacgtatctg agctctctct ggtcaatttt gaggaatcta agacattttt tggcttatat
841 aagctctga
//
fasta格式是一種基于文本用于表示核酸序列或多肽序列的格式。其中核酸或氨基酸均以單個(gè)字母來表示，且允許在序列前添加序列名及注釋。該格式已成為生物信息學(xué)領(lǐng)域的一項(xiàng)標(biāo)準(zhǔn)。
例如：
>NM_213806
ATGCAGCAGCCCTTCAATTACCCATACCCCCAAATCTTCTGGGTGGACAGCAGTGCTACC
TCTCCCTGGGCCTCCCCAGGCTCAGTCTTCCCCTGTCCAGCTTCTGTGCCAGGAAGGCCA
GGGCAAAGGAGGCCACCACCACCACCGCCGCCACCGCCACCACCACCAACACTCCTGCCA
TCAAGACCGCTGCCTCCACTGCCACCGCCATCTCTGAAGAAGAAGAGGGACCACAATGCA
GGCCTGTGTCTCCTTGTGATGTTCTTCATGGTTCTGGTGGCCCTGGTTGGATTGGGGCTG
GGGATGTTTCAGCTCTTCCACCTACAGAAGGAGCTGACTGAACTCAGAGAGTCTGCCAGC
CAAAGGCATACAGAATCATCTTTGGAGAAGCAAATAGGTCACCCCAATCTACCCTCTGAG
AAAAAGGAGCTGAGAAAAGTGGCCCACTTAACAGGCAAGCCTAACTCAAGATCCATCCCT
CTGGAATGGGAAGACACCTATGGAATTGCCTTGGTCTCTGGGGTGAAGTATATGAAGGGC
AGCCTTGTGATCAATGACACTGGGCTGTATTTTGTGTATTCCAAAGTGTACTTCCGGGGT
CAGTACTGCAACAACCAGCCCCTGAGTCACAAGGTATACACAAGGAACTCTAGGTATCCC
CAGGACCTGGTGCTGATGGAGGGAAAGATGATGAACTATTGCACTACTGGCCAAATGTGG
GCCCGCAGCAGCTACCTGGGGGCTGTGTTCAATCTCACCAGCGCTGACCATTTATATGTC
AACGTATCTGAGCTCTCTCTGGTCAATTTTGAGGAATCTAAGACATTTTTTGGCTTATAT
AAGCTCTGA
處理步驟
- 將文件夾下gb文件批量讀取
- 將各個(gè)gb文件中的登錄號(hào)和具體序列抽提出來，并寫入fasta文件
- 將fasta文件進(jìn)一步處理去掉換行符，使一個(gè)完整的序列中沒有換行符
- 將所有處理好的fasta文件存入一個(gè)新建的子文件夾中
Python腳本代碼如下：
#!/usr/bin/env python
# -*- encoding: utf-8 -*-
'''
@File : gb2fasta.py
@Time : 2020/07/04 14:15:13
@Author : Ai
@Version : 1.0
@Contact : aqy0716@163.com
@License : (C)Copyright 2020 SCAU
@Desc : 將gb文件轉(zhuǎn)換為fasta文件，同時(shí)轉(zhuǎn)成無換行符，最后存入新的子文件夾中
'''
# here put the import lib
import os
import shutil
def gb2fasta(path):
# 從gb文件中抽取登錄號(hào)和具體序列信息存入同名fasta文件
# 讀取文件夾相關(guān)信息
for root,dirs,files in os.walk(path):
for file in files:
# 打印文件所屬目錄
print(root + '\\' + file)
# 獲取文件路徑
path_gb = os.path.join(root,file)
flag=0
if path_gb[-2:] == 'gb':
# 打開新建fasta文件準(zhǔn)備寫入
fasta = open (path_gb[:-2]+'fasta','w')
# 打開gb文件，準(zhǔn)備讀取序列信息并寫入fasta文件
with open (path_gb,'r') as f:
# 逐行掃描
for line in f:
# 如果是ACCESSION行，則寫入fasta文件作為序列標(biāo)題
if line[0:9] =='ACCESSION':
fasta.writelines('>'+line.split()[1]+'\n')
# 如果是ORGIN行，代表是序列
elif line[0:6] =='ORIGIN':
flag = 1
elif flag == 1:
#通過空格符（空格 換行 制表）對(duì)字符串進(jìn)行切片
s = line.split()
#非空切片字符打印
if s != []:
#print(s)
#去掉列表首個(gè)元素（數(shù)字序號(hào)）后，連接所有元素即為完整序列按行寫入fasta文件
seq=''.join(s[1:])
fasta.writelines(seq.upper()+'\n')
fasta.close()
def multi2single(path):
#此函數(shù)功能為： 將多行序列轉(zhuǎn)換為單行序列（即去掉換行符），成為標(biāo)準(zhǔn)fasta文件
for root,dirs,files in os.walk(path):
for file in files:
path_full = os.path.join(root,file)
# 有fasta 且不為 single.fasta，才進(jìn)行單行轉(zhuǎn)換，否則會(huì)重復(fù)創(chuàng)建文件夾
a = path_full[-5:] == 'fasta'
if path_full[-12:] == 'single.fasta':
b = True
else:
b = False
b = bool(1-b)
if a & b :
fr=open(path_full, 'r')
fw=open(path_full[:-6]+'_single.fasta', 'w')
seq={}
for line in fr:
if line.startswith('>'): #判斷字符串是否以‘>開始’
name=line.split()[0] #以空格為分隔符，并取序列為0的項(xiàng)。
seq[name]=''
else:
seq[name]+=line.replace('\n', '')
fr.close()
for i in seq.keys():
fw.write(i)
fw.write('\n')
fw.write(seq[i])
fw.write('\n')
fw.close()
def copy2subdir(path):
# 將生成的_single.fasta文件存入新的子文件夾.\singl_fasta\中
root1 = path
for root,dirs,files in os.walk(path):
subdir = os.path.join(root1,'singl_fasta\\')
for file in files:
oldfile = os.path.join(root1,str(file))
newfile = os.path.join(root1,'singl_fasta\\',str(file))
if not os.path.exists(subdir):
os.makedirs(subdir)
print("目錄創(chuàng)建成功！")
if oldfile[-12:] == 'single.fasta':
if not os.path.exists(newfile):
shutil.copyfile(oldfile , newfile)
print("\n你的fasta文件保存在: " + subdir + "文件夾下\n")
if __name__ == "__main__":
path = input("請輸入路徑") #此處輸入 D:\docu\gb2fasta
gb2fasta(path)
multi2single(path)
copy2subdir(path)
運(yùn)行情況
PS D:\vscode_python_magic> & d:/ruanjiancangku/python_projectkotin/venv/Scripts/python.exe d:/vscode_python_magic/實(shí)驗(yàn)室-magic/序列處理/gb2fasta.py
D:\docu\gb2fasta\1-FASLG-swine-849bp-NM_213806.fasta
D:\docu\gb2fasta\1-FASLG-swine-849bp-NM_213806.gb
['1', 'atgcagcagc', 'ccttcaatta', 'cccatacccc', 'caaatcttct', 'gggtggacag', 'cagtgctacc']
['61', 'tctccctggg', 'cctccccagg', 'ctcagtcttc', 'ccctgtccag', 'cttctgtgcc', 'aggaaggcca']
['121', 'gggcaaagga', 'ggccaccacc', 'accaccgccg', 'ccaccgccac', 'caccaccaac', 'actcctgcca']
['181', 'tcaagaccgc', 'tgcctccact', 'gccaccgcca', 'tctctgaaga', 'agaagaggga', 'ccacaatgca']
['241', 'ggcctgtgtc', 'tccttgtgat', 'gttcttcatg', 'gttctggtgg', 'ccctggttgg', 'attggggctg']
['301', 'gggatgtttc', 'agctcttcca', 'cctacagaag', 'gagctgactg', 'aactcagaga', 'gtctgccagc']
['361', 'caaaggcata', 'cagaatcatc', 'tttggagaag', 'caaataggtc', 'accccaatct', 'accctctgag']
['421', 'aaaaaggagc', 'tgagaaaagt', 'ggcccactta', 'acaggcaagc', 'ctaactcaag', 'atccatccct']
['481', 'ctggaatggg', 'aagacaccta', 'tggaattgcc', 'ttggtctctg', 'gggtgaagta', 'tatgaagggc']
['541', 'agccttgtga', 'tcaatgacac', 'tgggctgtat', 'tttgtgtatt', 'ccaaagtgta', 'cttccggggt']
['601', 'cagtactgca', 'acaaccagcc', 'cctgagtcac', 'aaggtataca', 'caaggaactc', 'taggtatccc']
['661', 'caggacctgg', 'tgctgatgga', 'gggaaagatg', 'atgaactatt', 'gcactactgg', 'ccaaatgtgg']
['721', 'gcccgcagca', 'gctacctggg', 'ggctgtgttc', 'aatctcacca', 'gcgctgacca', 'tttatatgtc']
['781', 'aacgtatctg', 'agctctctct', 'ggtcaatttt', 'gaggaatcta', 'agacattttt', 'tggcttatat']
['841', 'aagctctga']
['//']
D:\docu\gb2fasta\2-LTA-swine-1584bp-NM_214453.fasta
D:\docu\gb2fasta\2-LTA-swine-1584bp-NM_214453.gb
['1', 'agaaaggggc', 'ccacaggggt', 'cccgcacagc', 'aggtgagact', 'ctcccacccc', 'atctcctagg']
['61', 'gctgtccggg', 'tgctggactc', 'ccccctcact', 'tcggtccctc', 'cgcccgctcc', 'ctggccttcc']
['121', 'tgcccctcct', 'gcatcttcac', 'cccggcctgg', 'gccttggtgg', 'gtttggtttt', 'ggtttgttct']
['181', 'ctctgattct', 'ttatctgtca', 'ggctctttct', 'agctctcaca', 'cactctgatc', 'cctctctgtt']
['241', 'cccttcccat', 'ctctgtttct', 'ctctgggtct', 'ccccctgctc', 'acctcgggat', 'ttccctgagt']
['301', 'gcctctggtc', 'cccttctctg', 'tctggcgccc', 'cgtctcttgt', 'ctctcggggt', 'ggctgtctcc']
['361', 'gagggcagga', 'ggccttcttc', 'cgcaggtgcc', 'ccgccccgct', 'cactgtctct', 'ctccccccac']
['421', 'aggttttccc', 'catgacacca', 'cctggacgcc', 'tctacctccg', 'gagggtgtgc', 'agcaccccca']
['481', 'tcctcctcct', 'cctggggctg', 'ctgctggccc', 'tgccgcccga', 'ggcccagggg', 'ctccctggcg']
['541', 'tcggcctccc', 'accctcagct', 'gcacagcctg', 'cccatcagca', 'ccccccaaag', 'cacttggcca']
['601', 'gaggcaccct', 'caaacctgcc', 'gctcacctcg', 'ttggagaccc', 'cagcaccccg', 'gactcactgc']
['661', 'gctggagagc', 'gaacacggat', 'cgtgccttcc', 'tccgccatgg', 'cttcttgctg', 'agcaacaact']
['721', 'ccctgctggt', 'ccccaccagt', 'ggcctctact', 'ttgtctactc', 'ccaggtcgtc', 'ttctccgggg']
['781', 'aaggctgctt', 'ccccaaggcc', 'acccccaccc', 'ctctctacct', 'ggcccacgag', 'gtccagctct']
['841', 'tctcctccca', 'gtaccccttc', 'cacgtgccgc', 'tcctcagcgc', 'tcagaagtcc', 'gtgtgccccg']
['901', 'ggccacaggg', 'accttgggtg', 'cgctctgtgt', 'accagggggc', 'tgtgttcctg', 'ctcacccagg']
['961', 'gagatcagct', 'gtccacacac', 'acagacggca', 'ccccccacct', 'gctcctcagc', 'cccagtagcg']
['1021', 'tcttctttgg', 'agccttcgct', 'ctatagaaga', 'atccagaaag', 'aaaaaaattg', 'gtttcaaggc']
['1081', 'cttctcccct', 'tttcacctcc', 'cttatgacca', 'cttcggaggt', 'caccgcgcct', 'ctcctctgac']
['1141', 'aatttccaac', 'agtctcatct', 'tcccccacgc', 'tcagcacctg', 'gagcttctgt', 'agaaggaatt']
['1201', 'ctaggcacct', 'cgggggaact', 'ggaaccaccc', 'cggatgctct', 'gctgaggatc', 'tgaatgcccg']
['1261', 'cctggagccc', 'ttcccctgtc', 'ctgcccgtct', 'aggggccctc', 'gtccaggacg', 'tggaagggaa']
['1321', 'gctgacccat', 'gagggacttt', 'gaacggatga', 'ccggagcggt', 'gtgggggggt', 'tatttatgaa']
['1381', 'ggggaaaatt', 'aaattattta', 'tttatggagg', 'atggagagaa', 'gggaatcaca', 'gagggatgtc']
['1441', 'agaagagtgt', 'gacacatgtg', 'cccaagagat', 'aaagtgacag', 'aaggcatggg', 'ctccagatga']
['1501', 'cccggccaga', 'gagggcaaag', 'tggctcagga', 'aggggctgct', 'tgactggagg', 'ctcatgagga']
['1561', 'gacggctgac', 'cctcgatgaa', 'accc']
['//']
D:\docu\gb2fasta\3-LTB-swine-950bp-NM_001185138.fasta
D:\docu\gb2fasta\3-LTB-swine-950bp-NM_001185138.gb
['1', 'tcggatgggg', 'gcaccggggc', 'tggagggccg', 'gggtaggagg', 'ccccagggga', 'agggatgcct']
['61', 'cctgctggcc', 'gtggcagggg', 'ccacttccct', 'ggtgaccctc', 'ctgctggccg', 'tgcctatcac']
['121', 'ggtcctggct', 'gtgctggcct', 'tggtgcccca', 'ggagcaggga', 'gaactggtaa', 'cagggaccgc']
['181', 'tgacccaggc', 'acccaggcgg', 'aggcccagca', 'gcgattggag', 'tccaaggaga', 'cgccagagga']
['241', 'ggaggcagaa', 'acagatctca', 'gccccaggct', 'cccagctgcc', 'cacctcattg', 'gcgcttggat']
['301', 'cacgggtcag', 'gggctaggct', 'gggaggcgaa', 'gaaagaagag', 'gcgtttctga', 'ggagcgggac']
['361', 'gcagttctct', 'ggcgcggagg', 'gcctggccct', 'cccgcaggac', 'ggcctctact', 'acctctactg']
['421', 'tcacgtcggc', 'taccggggcc', 'gggcacctcc', 'tcccggcggg', 'gaccccctgg', 'accgctcggt']
['481', 'cacgctgctc', 'agccggctgt', 'accgggcggg', 'gggcgcctac', 'ggaccgggga', 'ctcccgagct']
['541', 'gctgctggag', 'ggcgcggaga', 'ctgtgactcc', 'ggtcttggac', 'cccagtcgga', 'ggcacgagta']
['601', 'cgggcccctc', 'tggtacacga', 'gcgtggggtt', 'cggtggcctg', 'gtgcagctcc', 'ggaggggcga']
['661', 'gagggtgtac', 'gttaatatca', 'gtcaccccga', 'tatggtggat', 'tacaggagag', 'gaaagacctt']
['721', 'cttcggggcg', 'gtgatggtgg', 'gctgaggact', 'gtccgcggcc', 'cgagaggacc', 'actgcatggt']
['781', 'gggagtgtgt', 'cgatggatca', 'agcccagaca', 'cggggtccca', 'gacaccaggc', 'cagacaccat']
['841', 'ggccgtgggg', 'aaaatgcagg', 'agatcgtgtg', 'gaaaactgat', 'tttgagcctg', 'atgaaaataa']
['901', 'agaatgtaaa', 'agctttaatn', 'gctgcccatg', 'ccaaaaaaaa', 'aaaaaaaaaa']
['//']
D:\docu\gb2fasta\4-TNF-swine-1666bp-NM_214022.fasta
D:\docu\gb2fasta\4-TNF-swine-1666bp-NM_214022.gb
['1', 'cccagagtga', 'ggacaccagg', 'ggaccagcca', 'ggagagagac', 'aagccatctc', 'caggaccccc']
['61', 'tagaaataac', 'ctctcagaag', 'acacaccccc', 'gaacaggcag', 'ccggacgact', 'ctctccctct']
['121', 'cacacgctgc', 'cccggggcgc', 'caccatctcc', 'cagctggacc', 'tgagcccctc', 'tgaaaaagac']
['181', 'accatgagca', 'ctgagagcat', 'gatccgagac', 'gtggagctgg', 'cggaggaggc', 'gctcgccaag']
['241', 'aaggccgggg', 'gcccccaggg', 'ctccaggagg', 'tgcctgtgcc', 'tcagcctctt', 'ctccttcctc']
['301', 'ctggtcgcag', 'gagccaccac', 'gctcttctgc', 'ctactgcact', 'tcgaggttat', 'cggcccccag']
['361', 'aaggaagagt', 'ttccagctgg', 'ccccttgagc', 'atcaaccctc', 'tggcccaagg', 'actcagatca']
['421', 'tcgtctcaaa', 'cctcagataa', 'gcccgtcgcc', 'cacgttgtag', 'ccaatgtcaa', 'agccgaggga']
['481', 'cagctccaat', 'ggcagagtgg', 'gtatgccaat', 'gccctcctgg', 'ccaacggcgt', 'gaagctgaaa']
['541', 'gacaaccagc', 'tggtggtgcc', 'gacagatggg', 'ctgtacctca', 'tctactccca', 'ggtcctcttc']
['601', 'aggggccaag', 'gctgcccttc', 'caccaacgtt', 'ttcctcactc', 'acaccatcag', 'ccgcatcgcc']
['661', 'gtctcctacc', 'agaccaaggt', 'caacctcctc', 'tctgccatca', 'agagcccttg', 'ccagagggag']
['721', 'acccccgagg', 'gggccgaggc', 'caagccctgg', 'tacgaaccca', 'tctacctggg', 'aggggtcttc']
['781', 'cagctggaga', 'aggatgatcg', 'actcagtgcc', 'gagatcaacc', 'tgcccgacta', 'tctggacttt']
['841', 'gctgaatctg', 'ggcaggtcta', 'ttttgggatc', 'attgccctgt', 'gagggggcag', 'gacatccgtt']
['901', 'ccctcccctg', 'tccatccctt', 'tattatttta', 'ctccttcaga', 'ccccctcacg', 'tccttctggt']
['961', 'ttagaaagag', 'aatgaggggc', 'tggggactgg', 'gctccaagct', 'taaaacttta', 'aacaacaaca']
['1021', 'gcaacactta', 'gaaatcaggg', 'attcagggat', 'gtgtggcctg', 'gacaaccagg', 'cactgaccac']
['1081', 'caccaagaat', 'tggaactggg', 'gcttccagac', 'tcgctggggt', 'ccttgggttt', 'ggattcctgg']
['1141', 'atgcaacctg', 'ggacatctgg', 'aatgtggctg', 'ccagggaagc', 'ttgggttcca', 'atcggaatac']
['1201', 'ttcagaacat', 'tccttgagaa', 'gatttcacct', 'caatcttgat', 'gactttttag', 'gcttcccttt']
['1261', 'cttccaattt', 'tccagacttc', 'cctgggatgg', 'ggagcccagc', 'cccaaacccc', 'acaggccagc']
['1321', 'tccctcttat', 'ttatatttgc', 'acttggcatt', 'attatttatt', 'tatttattta', 'ttatttattt']
['1381', 'actagtgaat', 'gtatttattc', 'aggagggcga', 'ggtgtcctgg', 'gagacccagc', 'ataagggctg']
['1441', 'ccttggttca', 'gatgtgtttt', 'ctgtgaaaac', 'ggagctgaac', 'tgtaggttgc', 'tcccacctgg']
['1501', 'cctcctagcc', 'tctgtgcctc', 'cttttgctta', 'tgtttttaaa', 'aacaaatatt', 'tatctgatcg']
['1561', 'agttgtctaa', 'ataatgctga', 'tttggtgact', 'aacttgtcgc', 'tacatcgctg', 'aacctctgct']
['1621', 'ccccagggga', 'gttgtgtctg', 'taaccgccct', 'actggtcagt', 'ggcgag']
['//']
D:\docu\gb2fasta\5-TNFSF4-swine-549bp-NM_001025217.fasta
D:\docu\gb2fasta\5-TNFSF4-swine-549bp-NM_001025217.gb
['1', 'atggaagggg', 'tccaacccct', 'agatgaaaat', 'gtgggaaacg', 'caccaggacg', 'aagactcttg']
['61', 'aggaacaagc', 'tattgttggt', 'ggcctccgta', 'attcagggtc', 'tggggttgct', 'cctgtgtctc']
['121', 'acctacatct', 'gcctgcacct', 'ctatgctcag', 'gtgccatctc', 'agtaccctcc', 'aattcagagt']
['181', 'atcaaagtac', 'aatttaccaa', 'gtgtgaaaat', 'gataatggtt', 'tcatcatcac', 'accctcaagc']
['241', 'aaggatggaa', 'ccatgaaagt', 'gcaaaacaac', 'tcaatcatca', 'tcaactgtga', 'tgggttctat']
['301', 'ctcatctccc', 'tgaagggtta', 'cttttctcag', 'gagctcagcc', 'tcatgcttca', 'gtaccggaag']
['361', 'ggtcggaaac', 'ctctcttctc', 'cctgaacaag', 'gtcaagtctg', 'tggactctgt', 'cacagtagcc']
['421', 'gatctggctt', 'tcaaggacaa', 'ggtcttcctg', 'aacgtgacca', 'ctcatagtgc', 'ctcctgtgaa']
['481', 'gacattcagg', 'tgaatggtgg', 'ggaattgatt', 'ctcattcatc', 'aaaatcctgg', 'tggattctgt']
['541', 'gtctactga']
['//']
D:\docu\gb2fasta\6-TNFSF10-swine-1696bp-NM_001024696.fasta
D:\docu\gb2fasta\6-TNFSF10-swine-1696bp-NM_001024696.gb
['1', 'agcagtcaga', 'ccctgcctgg', 'accatggcgg', 'tgatgcagac', 'tccaggaggc', 'cccagccccg']
['61', 'ggcagacctg', 'tgtgttgatc', 'ctgatcttca', 'cagtgctcct', 'gcaagccctc', 'tgtgtggcct']
['121', 'tgacttacgt', 'gtacttcacc', 'aatgaactga', 'aacagatgca', 'ggacaagtac', 'tccaaaagcg']
['181', 'gtatagcttg', 'cttcttaaag', 'gaagatgaca', 'gtttctggga', 'tcccaccgat', 'gacgagagaa']
['241', 'tgctcagccc', 'ctgctggcag', 'gtgaagtggc', 'agctacgtca', 'gtttgtgaga', 'aagatgattt']
['301', 'tgagaaccta', 'tgaggaaacc', 'atttctacag', 'tttcagaaaa', 'gcaacaaggc', 'attcctcacc']
['361', 'tagaaagaga', 'aaaaggtcca', 'cagagagtgg', 'ctgctcacat', 'aactggaacc', 'agtaggaaaa']
['421', 'gaagcacatt', 'tccatctcta', 'agctccaaat', 'atgaaaaagc', 'tttgggccag', 'aaaataaact']
['481', 'cctgggaatc', 'atcaagaaaa', 'ggacattcat', 'tcttgaataa', 'ttttcacttg', 'aggaatggag']
['541', 'agctggttat', 'ccatcaaaca', 'gggttttact', 'acatctattc', 'ccaaacatac', 'tttcgatttc']
['601', 'aggaacctga', 'ggaaattttg', 'ggaacggttt', 'ctacagaagg', 'gaacagaaag', 'aaaaacaggc']
['661', 'aaatgataca', 'gtatatttac', 'aaatggacaa', 'gctatcctga', 'ccctatactg', 'ctgatgaaaa']
['721', 'gtgctagaaa', 'tagttgttgg', 'tctaaagatt', 'cagaatatgg', 'actctattcc', 'atctatcaag']
['781', 'gtggaatatt', 'tgagcttaag', 'gaagatgacc', 'gaatttttgt', 'ctctgttact', 'aatgagcaac']
['841', 'tgattgacat', 'ggaccaagaa', 'gccagttttt', 'tcggggcctt', 'tttaattggc', 'taaatgatct']
['901', 'gcagggaaaa', 'aaaccatgcc', 'ccagagtgac', 'tattcagagt', 'cgtatactgt', 'gaaaatattc']
['961', 'cagcagagcc', 'aataggttaa', 'ggcagcctga', 'gcaaagaggc', 'ctcaacccaa', 'aggctcaaca']
['1021', 'acacaagctt', 'tttggaaagt', 'gaaaagtgac', 'caattccttc', 'caggaaaatg', 'aaactgccaa']
['1081', 'gagaccttgt', 'ggagctctgc', 'ctgatgtcat', 'tttgctagta', 'aacatctaga', 'agatactctg']
['1141', 'tctccaaatt', 'tgtgtaacaa', 'ttaacacctc', 'ctgcctttat', 'catctaatcc', 'tgtgaagatt']
['1201', 'ctagaagaaa', 'gagtagtgat', 'ccatctcagg', 'tgggaataag', 'ggacaacatt', 'cccaaaacta']
['1261', 'aagagaaaag', 'ggcagcactg', 'aaaggtcaca', 'gtcaatatat', 'gcagtttcag', 'tacaaacata']
['1321', 'acaaattaaa', 'gctacgttta', 'gtggacaagg', 'agctacttct', 'gaatggtttg', 'tgcttttctc']
['1381', 'tactaaaaat', 'caggctggcc', 'aaaagcactc', 'agggtatttt', 'tgataaagga', 'ctctaaaata']
['1441', 'agtgataaag', 'tatggcgata', 'cctcagaaaa', 'ctaaatacag', 'aactaccaca', 'tgacccagca']
['1501', 'atcccactcc', 'tgtgcatata', 'tctggacaaa', 'actttccttg', 'aaaaagatac', 'attcatctct']
['1561', 'atgctcattg', 'cagcactatt', 'cacagtagcc', 'aagacatgga', 'aacacctata', 'tgtctatgaa']
['1621', 'tggatgaata', 'gattaagaag', 'gtgtgttatg', 'tatacatant', 'ggaatactgg', 'gaagccataa']
['1681', 'aaaggacaaa', 'gaggcc']
['//']
D:\docu\gb2fasta\7-TNFSF13b-swine-1013bp-NM_001097498.fasta
D:\docu\gb2fasta\7-TNFSF13b-swine-1013bp-NM_001097498.gb
['1', 'tctaggaggg', 'aaatggatga', 'ctccacgggg', 'gagcagtcac', 'gcctttcttg', 'ccttagcacg']
['61', 'agagaagaaa', 'tgaaactgaa', 'ggagacggtc', 'cccatcctcc', 'cccagaagga', 'aagcccctct']
['121', 'gtccgcatct', 'ccaaagatgg', 'gaagctgctg', 'gtcgtgaccc', 'tgctgctggc', 'cctgctgtcc']
['181', 'tgctgcctca', 'cggggatctt', 'tgcaccacca', 'gctccaaggg', 'agagcagctc', 'cattcaaagc']
['241', 'aacaggagta', 'agcgcgccgc', 'gcaggatgcg', 'gaggagacag', 'tcactcagga', 'ctgcttgcaa']
['301', 'ttgattgcag', 'acagtgacat', 'gcctactata', 'cgaaaaggag', 'cttatacatt', 'tgttccatgg']
['361', 'cttctcagct', 'ttaaaagagg', 'aagagcccta', 'gaagaaaaag', 'aaaataaaat', 'cgtggtcaaa']
['421', 'gaaacgggtt', 'acttttttat', 'atacggtcag', 'gttttataca', 'ccgataacac', 'ctttgccatg']
['481', 'gggcatctca', 'tacagaggaa', 'gaaagtccat', 'gtctttgggg', 'atgaactgag', 'tctggtgact']
['541', 'ttgttccgat', 'gtattcaaaa', 'tatgcctgaa', 'acactaccca', 'ataattcctg', 'ttattcagct']
['601', 'ggcattgcaa', 'agctggagga', 'aggagatgaa', 'ctccaactgg', 'caataccacg', 'tgaagacgct']
['661', 'aaaatatcac', 'gggatggaga', 'cggcacattt', 'tttggtgcat', 'tgaaacttct', 'gtgacctact']
['721', 'tacaccttgt', 'ttgtggctct', 'tgccctccct', 'ccctctgtac', 'ctctaaagag', 'aaaacactta']
['781', 'actggaaata', 'ccaaaagggg', 'aaaaaaaagt', 'agttaccata', 'gccttttctg', 'tgagctgttt']
['841', 'gttttggttt', 'gctgaaacta', 'gaccaaaaca', 'ggaaatttaa', 'cagacaacca', 'cagccaaagg']
['901', 'gtatcatgtg', 'aattacaaga', 'aatagagccc', 'atttaagaaa', 'aaatagaatt', 'agaaagactt']
['961', 'ttcactgtaa', 'tgccatgttg', 'aacagcttag', 'tcatagcttc', 'ttgtcttgga', 'gga']
['//']
D:\docu\gb2fasta\8-TNFSF18-swine-5005bp-XM_005667782.fasta
D:\docu\gb2fasta\8-TNFSF18-swine-5005bp-XM_005667782.gb
['1', 'gcattccatt', 'taacaaatga', 'aaaggctaag', 'gcataaagaa', 'ccaggagaga', 'gaaccggaga']
['61', 'tttcctcaat', 'tttagtgtag', 'taaatcaaca', 'gttttagtgc', 'taggaagttt', 'tttggaatag']
['121', 'agtgtaaact', 'cagatggtag', 'gacagggtgc', 'atgaaaatat', 'ccttttctat', 'gataacttta']
['181', 'tttctgtctg', 'atgtcagcat', 'tgaaatttca', 'gagtattaaa', 'atggtgaggt', 'atgaaaaaac']
['241', 'taagcttgtt', 'gttattgaca', 'tatttttaaa', 'aataaaattc', 'tagtaataca', 'ttactgtttt']
['301', 'ctagagatta', 'tctcagaatg', 'gacacattgt', 'ataccctagc', 'aattgatgaa', 'aatatttttc']
['361', 'ccaaaccatg', 'aaccccagca', 'tccttagctc', 'cctgacctac', 'tgcctcccac', 'aaacatgata']
['421', 'tttggagtat', 'gatagacctt', 'catcttgaat', 'ttcattcttt', 'ttcttacaaa', 'agtaattttc']
['481', 'ttatctggaa', 'ataatttgta', 'atgttgaata', 'gttccatagt', 'tcctcttgct', 'tcagaaaata']
['541', 'tttatttttc', 'ctcttaactt', 'cccttgttgg', 'gttttttttt', 'tttaaatcat', 'ctgtgtttgt']
['601', 'gttggcttct', 'agccctcagt', 'tccagcacct', 'ttggtctggt', 'gccaaatgtt', 'agtcagcact']
['661', 'taggctaaaa', 'gtatcgtttt', 'ccaacaccca', 'gatcagaagg', 'aaaactccgc', 'ctcttacacc']
['721', 'cactacttag', 'tgctatacta', 'caaaactgac', 'tagttgaatc', 'atgtgctcat', 'tacttctgaa']
['781', 'tttctgcttt', 'tcacaactct', 'cattcctgca', 'gagaatgagt', 'ttgagccaca', 'tggagaatat']
['841', 'gcctttaagc', 'cattcaagtc', 'ctcacgcagc', 'acagagacca', 'tcctggaagc', 'aatggctact']
['901', 'ctactcaaca', 'atagttattt', 'tgctattact', 'ttgctccttc', 'agtgcactaa', 'tcttaacttt']
['961', 'tctcccactc', 'aagacctcca', 'acaggccatg', 'tgtagcgaag', 'tttggaccat', 'taccttcaaa']
['1021', 'atggcaaatg', 'ccatctcctg', 'agccttcttg', 'tgtgaataag', 'acagatgatt', 'ggaggctgaa']
['1081', 'gatacttcag', 'aatggcttgt', 'atttaattta', 'tggccaagtg', 'gctcccaaca', 'cagcttacaa']
['1141', 'ggggcaagct', 'ccttttgagg', 'tgttgctacg', 'taggaatgaa', 'gaccccatac', 'aatctctaac']
['1201', 'gaacaattct', 'acagtccaga', 'atgtaggagg', 'ggcttatgaa', 'tttcatgctg', 'gagatgtaat']
['1261', 'agacttgata', 'ttcaatgctg', 'aacatcaggt', 'tctaaaaaat', 'aatacatact', 'gggggatctt']
['1321', 'tctgctagca', 'aatccccaat', 'tcatctccta', 'gagactcagt', 'taggtctcct', 'catcttcagc']
['1381', 'acatgcagag', 'atgccagtgc', 'ataggatgga', 'gaaggaagat', 'tttcaacaca', 'tacagttcat']
['1441', 'ctgggtatac', 'aaatcaacat', 'gaacagatct', 'cctctgcatg', 'tgaagcttca', 'tttctcctgc']
['1501', 'ttattgaatg', 'agactcagaa', 'agcactgaag', 'acatttggtt', 'acccctgatg', 'ttgggtcagc']
['1561', 'aaagacactt', 'tactagttca', 'tgataaaatg', 'aaaatgggtg', 'gctggaagac', 'aaaatctttt']
['1621', 'caaagtgtct', 'gtctaatcct', 'tgaacccctg', 'agtggaaaaa', 'tgaggtctat', 'tcccataata']
['1681', 'gccttatata', 'gcatgcaaaa', 'aaagaccagg', 'gcagtagcct', 'ggtcttgttc', 'ttatattctt']
['1741', 'ggactgtgga', 'ctgtttcaat', 'tcattcttcc', 'catattctca', 'tcttaggaga', 'cactcttaat']
['1801', 'aaaatgtagt', 'cagagtgggt', 'gtgtggccag', 'caacactcca', 'ttttggagtt', 'gatgagatta']
['1861', 'ggggatagag', 'aacactctta', 'ggaaatattg', 'ggacagaatt', 'tcagttggca', 'ttgaaatgga']
['1921', 'atgcacttta', 'ttcgggaatt', 'tcacttgatt', 'tcatcatcaa', 'gtgcagggtg', 'ctctataaaa']
['1981', 'cctgctggtc', 'aaaaggctag', 'ctttcaatct', 'tcacatagca', 'gttcatgaga', 'atttactggt']
['2041', 'gtatgtatct', 'aaccatgtca', 'atgacaaaga', 'gtaatcatta', 'gtagtaagat', 'ctaacccccc']
['2101', 'aaattggtat', 'taccagtact', 'gtactttgca', 'actgtgcaga', 'gccagctaaa', 'aatatgaaat']
['2161', 'cattacatga', 'caaagcactt', 'tcatatacca', 'catggcaact', 'cgatagattt', 'aatggggcag']
['2221', 'atatttttgg', 'cacaatttta', 'actatgagaa', 'tacagaggca', 'gatggaatag', 'aggtaacttg']
['2281', 'gttcagttca', 'tagaactagt', 'aattaacagg', 'cacctgggct', 'tcccactgta', 'ttatactata']
['2341', 'ttagctttac', 'gattggtatt', 'tctgctatca', 'tgttagaagc', 'ctataaactt', 'taacagattt']
['2401', 'aaaattttca', 'gacagtatat', 'tcccttttag', 'tccaacagca', 'attttttcct', 'ttctcagcaa']
['2461', 'atttcttttc', 'ttttctttgc', 'ctggagcagg', 'gtacccaggg', 'tgttattcaa', 'gacttactac']
['2521', 'aacttaatct', 'ccttccttac', 'tttggtcaaa', 'tgtgttaact', 'tccaaaaata', 'atgaataata']
['2581', 'ctcaattcag', 'ggacagtctg', 'ttaaattttt', 'ggactctgca', 'aaattaacta', 'gctgcttatg']
['2641', 'ggttgttatt', 'aaaaggtatg', 'taggtaatgt', 'gattacatga', 'aaacccaatt', 'taaaatattt']
['2701', 'atggatattt', 'gtaaaaaatc', 'tacattatgt', 'taattaatag', 'tatcaccatt', 'aaaaactaat']
['2761', 'ttaagaatat', 'ttgtattgta', 'tgtaagaaaa', 'actgcttgga', 'agcagactaa', 'gcctgaggcc']
['2821', 'aagatgcctc', 'atagtatgtc', 'tttttttttt', 'tttttttaaa', 'tacatctgct', 'gagcagctgt']
['2881', 'agggacaaag', 'actggggtac', 'ctggttcctc', 'ttgtatttgt', 'gtatcatctc', 'aggaaattaa']
['2941', 'agttacataa', 'catacatata', 'tttatggaaa', 'cgtggtattg', 'atgttaactt', 'ataagcagta']
['3001', 'gtgtgctgga', 'gtgggctagc', 'actagctcag', 'gagagctgtt', 'aaatttttat', 'taattgtgta']
['3061', 'gtctggttat', 'taaatcatta', 'tccttgaaat', 'tggccatggt', 'aggacaattt', 'ataccatgtg']
['3121', 'aattagcaaa', 'tgctacaaat', 'cagggctttt', 'ctttttggaa', 'agcccatgca', 'ccagcacacc']
['3181', 'actgtttata', 'aaactcttct', 'taatgactcc', 'tctcagcccc', 'tgcctcagta', 'ttacaacagt']
['3241', 'caaggcaggc', 'aaggaaagtg', 'tcttactctc', 'agcaaaagcc', 'ccacagataa', 'atcatttctc']
['3301', 'agggcaggtg', 'gaggaatcta', 'cagctgtaac', 'cagatagata', 'gctaccaaca', 'tatgaccttt']
['3361', 'gaatttccct', 'agtgttgaaa', 'tttcaggctt', 'tgttttcaat', 'gtatactctg', 'ttcccttgtt']
['3421', 'tcttcaaaac', 'agtgtttata', 'ttttaaactg', 'acaataaaat', 'gtttgtacat', 'gggctgtagc']
['3481', 'tgatttatct', 'atgggttatc', 'tgacaagagt', 'tagtgatcta', 'gagaatgtgc', 'atcagagaaa']
['3541', 'tccttgtcca', 'tatgaaacac', 'tgctgagaga', 'gtgagaggca', 'aaagtcagag', 'ctgggggttt']
['3601', 'tgtcccaatt', 'tctgatgggt', 'taggcaaata', 'atttcactag', 'ctgactttca', 'cccacctcct']
['3661', 'caactctacc', 'ccacccatcc', 'accctcacca', 'tcaaagccat', 'catcaaccct', 'ctcatactaa']
['3721', 'ttgtttttac', 'cttctatttt', 'gcctcatcaa', 'agctgacctg', 'gagtgctttt', 'tatcactgtg']
['3781', 'ggttaagtct', 'ctacttctga', 'agatgacagt', 'taattgatca', 'cagtcctgtg', 'tatttgatgg']
['3841', 'aacagataaa', 'ctccttagat', 'gcattatatc', 'tttgcaactt', 'ggagataaaa', 'ttcctgtctt']
['3901', 'tgtgtgttct', 'tcctcaaaga', 'agcaattttc', 'attggctggg', 'ggtgctagta', 'cgttttttat']
['3961', 'tagtgagaag', 'gagaagcgaa', 'aacccaattg', 'acttatgggc', 'tagtggtctg', 'aggcttacct']
['4021', 'tcaagcagag', 'tttttcaaag', 'tgctatctat', 'ggactctaca', 'ttagatttgc', 'ccagtatact']
['4081', 'tggccaaaaa', 'ccaaagaaaa', 'accccaaaga', 'taccttgacc', 'tcattcatag', 'tccactaaat']
['4141', 'aaatgtttct', 'aggagtgagg', 'tctaggaatc', 'aaatttgtaa', 'tgagctctga', 'cataggttat']
['4201', 'tcttatacta', 'cagtttaaaa', 'accactgctt', 'tatggaatgc', 'aattttgaat', 'ctcagcagaa']
['4261', 'tgtttctcca', 'aatatttaaa', 'aacagtcaaa', 'gcttataaac', 'caatgaagtt', 'aaaaagcaaa']
['4321', 'gcaaaaagca', 'aaaccaaaat', 'tggtattcat', 'gagacttacc', 'ataagccatc', 'tctcatcaca']
['4381', 'aacaatggcc', 'ttctatcctc', 'tatttcagtt', 'tgaaaaaaag', 'gtcaggctct', 'tcactttcca']
['4441', 'taatctaatg', 'gcaactttgc', 'cagttgaatg', 'ctggcaatcc', 'atagcatatg', 'atccttgtca']
['4501', 'taatcagtac', 'tccattatct', 'acctgtatgc', 'tgtcaactct', 'caaatactta', 'tctctagcca']
['4561', 'aagttgttct', 'cctttaagtc', 'ttataactcc', 'acctgtctgc', 'acagtaattt', 'cacattggac']
['4621', 'atacaatgga', 'catctcaaat', 'tcaacttacc', 'ccaaatgaat', 'cttttaaggt', 'ttcaactaaa']
['4681', 'gcagcttttt', 'tcgaatccct', 'cctcatttca', 'attgtatctc', 'atgagccaaa', 'aacctctgaa']
['4741', 'tcatggagtc', 'attcctgact', 'tctatcttta', 'atctgtttaa', 'tcttaggaaa', 'actattatct']
['4801', 'ttactttcag', 'agaatctgac', 'ctctttttgg', 'ctatggctct', 'tgtctttgga', 'ccaatatttt']
['4861', 'tttcagtatc', 'ttcataactt', 'attacagtat', 'ctacatactt', 'attacattca', 'ggatttttgg']
['4921', 'actagaaact', 'gaattttatc', 'aaagtcttag', 'agtttatata', 'tctctcatat', 'atgaaaaacc']
['4981', 'acagctaaca', 'tcattcacaa', 'tggtg']
['//']
D:\docu\gb2fasta\gb2fasta.py
目錄創(chuàng)建成功！
你的fasta文件保存在: D:\docu\gb2fasta\singl_fasta\文件夾下
程序運(yùn)行前 D:\docu\gb2fasta\ 文件夾下 文件
運(yùn)行前
程序運(yùn)行后 D:\docu\gb2fasta\ 文件夾下 文件
運(yùn)行后
程序運(yùn)行后 D:\docu\gb2fasta\ singl_fasta\ 文件夾下 文件
保存fasta文件的文件夾
