求解答：輸入python figaro.py -i /media/a/FA6C7F276C7EDE37/16s/data -o /media/a/FA6C7F276C7EDE37/16s/data -f 1 -r 1 -a 465 -F zymo
出現(xiàn)報(bào)錯(cuò)：Traceback (most recent call last):
File "figaro.py", line 213, in <module>
resultTable, forwardCurve, reverseCurve = figaroSupport.trimParameterPrediction.performAnalysisLite(parameters.inputDirectory.value, parameters.minimumCombinedReadLength.value, subsample = parameters.subsample.value, percentile = parameters.percentile.value, forwardPrimerLength=parameters.forwardPrimerLength.value, reversePrimerLength=parameters.reversePrimerLength.value, namingStandardAlias=fileNamingStandard)
File "/home/a/figaro/figaroSupport/trimParameterPrediction.py", line 436, in performAnalysisLite
forwardReadLength, reverseReadLength = checkReadLengths(fastqList)
File "/home/a/figaro/figaroSupport/trimParameterPrediction.py", line 398, in checkReadLengths
raise fastqHandler.FastqValidationError("Unable to validate fastq files enough to perform this operation. Please check log for specific error(s).")
figaroSupport.fastqHandler.FastqValidationError: Unable to validate fastq files enough to perform this operation. Please check log for specific error(s).
看了log文件是空白的，請(qǐng)問這是fastq文件內(nèi)容有問題么?
微生物多樣性qiime2分析流程(3) 使用figaro來得到dada2正反向截?cái)鄥?shù)dada2插件對(duì)原始數(shù)據(jù)paired-end-demux.qza進(jìn)行質(zhì)量過濾需要兩個(gè)參數(shù)，trunc-len-f和trunc-len-r。這個(gè)想法是通過盡可能多地刪除較低質(zhì)量...