產(chǎn)品簡(jiǎn)介

RFectPM原代細(xì)胞siRNA轉(zhuǎn)染試劑是在RFect siRNA轉(zhuǎn)染試劑的基礎(chǔ)上加以改進(jìn)研發(fā)成功的專門(mén)用于原代細(xì)胞小核酸轉(zhuǎn)染的試劑。RFectPM可用來(lái)轉(zhuǎn)染siRNA、antisense RNA、microRNA等200bp以內(nèi)的小分子RNA和DNA。RFectPM使用極其簡(jiǎn)便，先將siRNA與RFectPM室溫混合，再將siRNA-RFectPM混合物直接加入含培養(yǎng)基的細(xì)胞，血清對(duì)轉(zhuǎn)染效果沒(méi)有影響，不必刻意添加或更換培養(yǎng)液。有關(guān)RFectPM原代細(xì)胞siRNA轉(zhuǎn)染試劑的材料合成和試劑配制我們已申請(qǐng)了國(guó)際專利，并通過(guò)PCT覆蓋國(guó)際上多個(gè)國(guó)家和地區(qū)。?

產(chǎn)品特點(diǎn)

卓越的原代細(xì)胞轉(zhuǎn)染性能：原代細(xì)胞轉(zhuǎn)染陽(yáng)性率一般在80%以上，基因敲除效果明顯，肝細(xì)胞LaminA/C基因敲除效率在90%以上；

極低的細(xì)胞毒性使實(shí)驗(yàn)結(jié)果更為客觀：轉(zhuǎn)染細(xì)胞死亡率不到10%；

低濃度siRNA轉(zhuǎn)染獲得高基因抑制水平；

轉(zhuǎn)染細(xì)胞范圍廣泛，絕大多數(shù)原代細(xì)胞都能獲得比較理想的轉(zhuǎn)染結(jié)果。對(duì)一些活體寄生蟲(chóng)幼蟲(chóng)，RFectPM也能獲得較為理想的轉(zhuǎn)染結(jié)果。

Fig.1. Superior knockdown with RFectPM?transfection reagent on difficult transfection primary cells.?Different primary cells?were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFectPM?.In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR. ??

Fig.2.?RFectPM?transfection reagent offers high level of gene knockdown with low concentration of siRNA. Lamin A/C siRNA-RFectPM transfection complexes were prepared in 96-well plates,and then bovine chondrocytes were added to each well.?